Composite

Part:BBa_K3247008

Designed by: Stephanie Trejo Corona   Group: iGEM19_Rice   (2019-10-16)


Para-B0034-otsBA-L3S1P00

This cassette contains a Pcon-araC promoter, an Elowitz RBS (BBa_B0034), the genes otsB and otsA. he otsA gene produces the trehalose-6-phosphate synthase enzyme. The otsB gene produces the trehalose phosphatase enzyme. These enzymes in sequence convert 2 glucose molecules into a dissacharide called trehalose in the following two reactions: UDP-glucose + D-glucose-6-phosphate -> UDP + a,a-trehalose-6-phosphate a,a-trehalose-6-phosphate + H2O -> a,a-trehalose + phosphate

Trehalose synthase protects plants against salinity and desiccation. By upregulating trehalose production, we hope to confer drought resistance to plants.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1991
    Illegal BamHI site found at 26
    Illegal BamHI site found at 488
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None